| 1. | Minimum dose of protection of recombinant pseudorabies virus rprv - gp5 against pseudorabies in sheep
对伪狂犬病最小免疫剂量的测定 |
| 2. | A survey on the carrier of pseudorabies virus in the immunized pigs on some breeder farms of guangxi
广西部分种猪场免疫猪伪狂犬病野毒感染情况调查 |
| 3. | Construction and immunogenicity of recombinant pseudorabies virus expressing the modified gp5m protein of porcine reproduction and respiratory syndrome virus
蛋白的重组伪狂犬病毒的构建及其在小鼠的免疫反应 |
| 4. | The reference min - a strain of pseudorabies virus ( prv ) was reproduced by adaption to pk - 15 cell and genome dna was extracted from the harvested infected culture after ultrasonification and centrifugation as template
将收获的细胞经- 20和37反复3次冻融后，超声粉碎，低速离心收集上清液，再以超速离收获病毒。 |
| 5. | Three pairs of primers were designed according to the sequences pulished by the genebank in order to amplifiy gd , ge and tk gene of the pseudorabies virus min - a strain . the gd , ge and tk gene were obtained by polymerase chain reaction ( pcr ) , and then cloned into the pgem - t easy vector
参考genebank收录的伪狂犬病病毒gd 、 ge 、 tk基因的序列设计了三对引物，对prvmin - a株进行了pcr扩增，扩增产物克隆于pgem - teasy载体。 |
| 6. | The purpose of this study is to construct a recombinant pseudorabies virus expressing ha gene of swine influenza virus and to develop specific diagnosis for differentiating the recombinant virus immunized animals and those naturally infected with prv or sfv
本试验的目的是以ge基因缺失的prv为载体构建可以表达sivha基因的重组病毒疫苗用于预防这两种病，同时，基于此重组病毒的特点，建立可以区分此重组病毒疫苗和prv及siv自然感染的鉴别诊断方法。 |
| 7. | Pseudorabies virus ( prv ) is the causative agent of aujeszky ' s disease , which results in significant losses in pig husbandry . thymidine kinase ( tk ) gene is one of the main virulent genes of prv , and it is essential to the propagation of prv in the nerve tissues , but dispensable for virus replication and infection in other tissues
伪狂犬病是由伪狂犬病病毒( pseudorabiesvirus , prv )引起的家畜和多种野生动物的一种以发热、奇痒、呼吸和神经系统疾病为特征的急性传染病，妊娠母猪可发生死胎和流产，是危害养猪业的一个重要传染病。 |
